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・ Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System, or One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (Cat. #RR096A/B)*2 *Follow manual of LightCycler (by Roche Diagnostics) for operation. 1. Prepare the following reagents on ice.. View and Download Applied Biosystems 7500 getting started manual online. Fast Real-Time PCR System. 7500 Laboratory Equipment pdf manual download. plus one endogenous control) is large • Optimization and validation requirements are reduced for singleplex experiments Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System

The primers and probe were synthesized in accordance with a previous report. Applied Biosystems ® StepOnePlusTM was used in this experiment. THUNDERBIRD ® Probe One-step qRT-PCR Kit was the only kit that detected less than 10 copies of RNA and showed wide-ranging quantitation. The results indicate that this kit is suitable for the highly

Applied biosystems step one plus manual

Reverse Transcription. Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs. The RT step may be performed on total RNA such that a global cDNA is produced that is representative of all of the RNA transcripts in the sample (usually via a two-step protocol), or in a gene-specific approach such that only the RNA of interest is

GoTaq® 1-Step RT-qPCR System The system enables detection of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq e.g., from Applied Biosystems. A separate tube of CXR Reference Dye is included with the GoTaq

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For detailed information on instrument setup and the calibration process, refer to the Applied Biosystems ™ StepOne and StepOnePlus Real-Time PCR Systems Installation, Networking and Maintenance User Guide (Pub. no. 4376782). Limited product warranty. Applied Biosystems StepOnePlus (for software version 2.0

Applied Biosystems StepOnePlus Real-Time PCR Machine 1. Introduction A StepOnePlus real-time PCR machine located at the main laboratory of IBEC at the Hèlix building is available for use by all IBEC researchers. The StepOnePlus System from Applied Biosystems supports any real-time PCR application, such as: • SNP genotyping

Reverse Transcription. Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs. The RT step may be performed on total RNA such that a global cDNA is produced that is representative of all of the RNA transcripts in the sample (usually via a two-step protocol), or in a gene-specific approach such that only the RNA of interest is

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Real-Time PCR ABI Prism 7500 (PSC 563, NSC 338) Real-Time PCR StepOne (PSC 563) Every time you use any of the qPCR instruments, you MUST sign the log book. Use Lab Commander to reserve a time. Each instrument has it's own log book. Use only optical grade tubes, flat top caps or plates, see below for order information.

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The One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) is designed for intercalator- ・ Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System, Please refer to the instruction manual for your real time PCR instrument to read about

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